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ID:833 Mycology Microscopy and culture
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DescriptionExamination of a specimen for fungi by a manual microscopy and culture method. Susceptibility testing may also be performed if indicated. Identification of yeasts, usually to species level using a combination of morphological and nutritional/enzymatic tests. Molecular identification may be carried out for those isolates which cannot be identified using phenotypic tests. Susceptibility testing of yeasts by CLSI M44A disc diffusion (fluconazole) or microbroth dilution (fluconazole, itraconazole, voriconazole, amphotericin B, flucytosine, caspofungin, posaconazole and ketoconazole). Specific antifungal(s) tested depend on the identity and source of the isolate and the clinical details supplied. Microbroth dilution testing is undertaken where indicated by isolate identity, disc diffusion results, or where requested specifically. The identity of the yeast isolate is always confirmed or carried out on isolates sent for sensitivity testing. Identification of moulds, usually to species level on the basis of macroscopic and microscopic morphology. Molecular identification may be carried out for those isolates which cannot be identified using phenotypic tests. Susceptibility testing of mould isolates may be indicated, though this is not done routinely. Please call to discuss.
IndicationSuspicion of superficial or systemic fungal infection.
Additional InfoThis type of investigation is indicated for skin hair and nail, but also any tissue, particularly sterile tissue, where a fungal infection is suspected.
Concurrent Testsna
Dietary Requirementsna
InterpretationMicroscopy: Negative, Positive for hyphae, yeast cells or fungal elements (cells which are clearly fungal but which may be yeasts or some form of conidia (spore)). Culture: Identity of fungus isolated usually to species level. No fungus isolated if negative. No significant growth if non-pathogenic fungus isolated. Sensitivities where performed are reported as "susceptible", "susceptible dose dependant", "intermediate" "resistant" or "non susceptible". MICs may be available for some isolates on request.

SampleSkin / Nail Scrapings
Tube Picture
Collection ConditionsMicroscopy cannot be performed on samples sent on charcoal swabs. Do not send e-swabs for Mycology only.
Min. Vol

Ref. Range (Male)
Ref. Range (Female)
Ref. Range (Paed)
Ref. Range Notes
Units - Not Defined -
IP Acute TAT24 days
IP Routine TAT24 days
GP Acute TAT24 days
GP Routine TAT24 days
Turnround Comment95% Turnaround times This test is carried out daily. 95% of Microscopy results are reported within 7 days. Results may be available considerably earlier than stated turnaround time.

Originally edited by : Richard Barton. Review due on 01/12/2018 13:04:05. Published By Kasia Okurowska on 01/12/2017 13:04:05.