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ID:803 Respiratory Virus PCR
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DescriptionDetection of respiratory pathogens by PCR in respiratory secretions is diagnostic for the presence of agents which may or may not be the cause of respiratory disease. This multiplex PCR detects Influenza viruses (A and B), RSV, Adenovirus, Metapneumovirus, Rhino virus, Parainfluenza viruses 1-4 and Mycoplasma pneumoniae.
Additional Info Respiratory secretions (NPA,BAL, Tracheal aspirate), nose and throat swabs are acceptable samples. Sputum will be tested on adults although swabs are preferred where upper respiratory tract symptoms predominate. . Swabs and transport media are available from Molecular lab, ext 28750 or by faxed form available on the web site. NPA is probably the best sample for young children but a nasopharyngeal swab is acceptable alternative or nose and throat swabs. Please do NOT use charcoal swabs or eswabs for this test. Samples must be in the laboratory before 09:30. Certain samples positive for influenza viruses may be referred to the Virus Reference Laboratory at Colindale for typing and/or confirmation.
Concurrent Testsna
Dietary Requirementsna
InterpretationSpecimens will be reported as positive or negative for each target.

SampleNPA / Sputum or Swab
TubeUTM / Plain Universal
Tube Picture
Collection ConditionsOptimal time of specimen collection is the acute phase of illness. Red top Copan UTM is the required transport medium. Please do NOT use pink top Copan e-swabs (liquid swabs with clear liquid) for virology. Do NOT send charcoal swabs for Virology
Min. Vol 250Ál fluid minimum

Ref. Range (Male)
Ref. Range (Female)
Ref. Range (Paed)
Ref. Range Notes
Units - Not Defined -
IP Acute TAT1 - 2 days
IP Routine TAT1 - 2 days
GP Acute TAT1 - 2 days
GP Routine TAT1 - 2 days
Turnround CommentSamples to be in Laboratory before 10:00 Weekdays and 9:00 Saturdays.

Originally edited by : D Bennett. Review due on 08/01/2020 12:08:49. Published By K Roberts on 08/01/2019 12:08:49.