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ID:63 Coeliac screen
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DescriptionCoeliac disease is a gluten sensitive enteropathy. Requests for a coeliac screen will be tested for IgA anti-tissue transglutaminase antibodies (tTG). If positive a follow up test of IgA anti-endomysial antibodies will be performed as a confirmatory test. Anti-gliadin Ab are no longer available.
IndicationInvestigation of coeliac disease and dermatitis herpatiformis. Chronic diarrhoea. Iron and folate deficiency. Reduced bone density.
Additional InfoPlease note that coeliac screening assays are sensitive to a gluten free diet - i.e. results are often negative in those excluding gluten from the diet. In patients with coeliac disease and total IgA deficiency, only IgG anitbodies are seen. Where IgA deficiency is detected, IgG endomysial Ab will be requested by the laboratory. An IgA verification bead is included in this assay and is therefore able to detect patients who are IgA deficient which makes the requesting of IgA unnecessary.
Concurrent Testsna
Dietary RequirementsNICE guidelines indicate gluten must be consumed for 6wks prior to testing.
InterpretationPositive anti- tTG and Endomysial are supportive of a diagnosis of Coeliac disease. Biopsy confirmation is still recommended. There is a good correlation with disease activity, with relapse or poor compliance with a gluten free diet often associated with a return of positive anti-tTG and endomysial Ab. Positive anti-tTG are present in 85-100% (sensitivity) of untreated adults with coliac disease (specificity is 71-100%). IgA anti-tTG is more sensitive but less specific than IgA endomysal Ab (sensitivity 85-100%; specificity 94-100%), and is therefore used as a confirmatory test. Joint BSPGHAN and Coeliac UK guidelines are now availble foe the diagnosis and management of coeliac disease in children (Published 2013).

DepartmentClinical Immunology
SampleBlood
TubeSerum Gel
Tube Picture
Collection ConditionsNo restrictions
Min. Vol2 mL
Freq.Weekdays

Ref. Range (Male)<15 U/mL
Ref. Range (Female)
Ref. Range (Paed)
Ref. Range NotesEndomysial Ab = Positive/Weakly positive/Negative
UnitsU/mL
IP Acute TAT- Contact Laboratory
IP Routine TAT7 days
GP Acute TAT- Contact Laboratory
GP Routine TAT7 days
Turnround CommentNA

Originally edited by : BD. Review due on 14/09/2018 14:26:04. Published By AM on 14/09/2017 14:26:04.