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ID:592 Bacterial 16S ribosomal DNA PCR
Search Links: General Info : Protocols : LabTestsOnline

DescriptionBacterial 16S ribosomal DNA PCR uses primers to conserved regions of the 16S ribosomal DNA of bacteria. In between the conserved regions DNA containing variable regions are also amplified. The variable regions are specific for particular species and therefore allow for molecular species identification. Some organisms cannot be identified unequivocally using this method but those instances are rare. 16S identification currently used when monobacterial sterile site infections are suspected (e.g. heart valves from infective endocarditis patients, CSF other than caused by N. meningitidis or S. pneumoniae). This test requires prior discussion with the medical microbiologist.
Indication
Additional Info
Concurrent Testsna
Dietary Requirementsna
Interpretation

DepartmentMicrobiology
Sample* Not Specified *
Tube
Tube Picture
Collection ConditionsSampling of sterile sites using aseptic techniques is paramount as any bacterial contamination will be detected due to the sensitivity of this test
Min. Vol
Freq.once

Ref. Range (Male)
Ref. Range (Female)
Ref. Range (Paed)
Ref. Range Notes
Units - Not Defined -
IP Acute TAT10 days
IP Routine TAT10 days
GP Acute TAT10 days
GP Routine TAT10 days
Turnround CommentNA

Originally edited by : D Bennett. Review due on 29/11/2017 12:06:48. Published By K Roberts on 29/11/2016 12:06:48.