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ID:526 Acanthamoeba investigation from keratitic eye lesions
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DescriptionAcanthamoeba keratitis is an infection of the cornea. Acanthamoeba are common in nature and are usually found in bodies of water (lakes,rivers) as well as domestic tap water, swimming pools, soil and air. Samples are referred from Microbiology for Molecular Detection by PCR to Micropathology Ltd. Turnaround time is 7 days.
Indication This test is indicated to rule out Acanthamoeba keratitis in contact lens wearers or in cases of keratitis that is unresponsive to therapy. Note that the initial cases of Acanthamoeba keratitis were not related to contact lenses.
Additional InfoRequest test via Order Comms. Adult Services J-O - Ophthalmology - Acanthamoeba Ensure sample is labelled. Please do not freeze, though, specimens may be refrigerated overnight.
Concurrent Testsna
Dietary Requirementsna
Interpretation A positive result will be issued as Acanthamoeba DNA detected. A negative result will be issued as Acanthamoeba DNA not detected

DepartmentMicrobiology
SampleCorneal scrape, corneal swabs and contact lens and can be used for this test. Aqueous samples can also be sent but if culture is also required the volume may not be sufficient
TubePlain Universal
Tube Picture
Collection ConditionsCorneal scrapings -use scalpel blade to take cells from the ulcer and place in 1ml of sterile saline in a sterile universal. Corneal swabs- use a cotton swab to firmly swab the ulcer and epithelium to remove cells. Place the swab in a sterile universal in 1ml and cut the shaft flush to the top of the bottle with sterile scissors. Contact lens - transfer to sterile universal containing sterile saline. Aqueous sample - Use a 1ml syringe with 30g needle. The test requires a minimum volume of 100 Ál fluid. After removing from eye withdraw plunger to draw the aqueous into the syringe from the needle before removing. Screw stopper on end and secure with surgial tape to stop it emptying. Samples must be labelled with patient name and numerical identifier. Use order comms labels whenever possible.
Min. Vol
Freq.

Ref. Range (Male)
Ref. Range (Female)
Ref. Range (Paed)
Ref. Range Notes
Units - Not Defined -
IP Acute TAT7 days
IP Routine TAT7 days
GP Acute TAT7 days
GP Routine TAT7 days
Turnround CommentNegative results may be available considerably earlier than stated turnaround time.

Originally edited by : D Bennett. Review due on 12/05/2018 11:44:45. Published By K Roberts on 12/05/2017 11:44:45.