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ID:28 Anti-nuclear antibodies (ANA)
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DescriptionAnti-nuclear antibody (ANA) testing can be used for the assessment of systemic autoimmune disease/ connective tissue disease.
IndicationSystemic autoimmune disease/connective tissue disease
Additional InfoA multiplex flow immunoassay using the Bioplex 2200 has replaced indirect immunofluorescence and anti-DNA and ENA Ab ELISA methods for the detection of ANA. NEW positive results will be confirmed using Indirect Immunofluorescence. The multiplex ANA screen can detect the presence of 12 different ANA components: dsDNA, Ro60, Ro52, La, Sm, SmRNP, RNP-68, CENP-B (centromere), Scl-70, Jo-1, chromatin and ribosomal P Ab. Detection of one or more of these autoantibody specificities can be useful in the diagnosis of systemic autoimmune/connective tissue diseases. If ANA by indirect immunofluoresce is required in addition to screening on the Bioplex analyser, you must first discuss this with the Consultant Immunologists.
Concurrent Testsna
Dietary Requirementsna
InterpretationThe presence of ANA occurs in a number of autoimmune conditions eg SLE, Systemic sclerosis, Sjogren's syndrome, dermato/polymyositis, and mixed connective tissue disease. Different autoantibody specificities are associated with different connective tissue diseases. Results from the ANA screen should always be interpreted in the context of relevant clinical signs and symptoms. Please see pathology web pages for further information http://www.pathology.leedsth.nhs.uk/pathology/ClinicalInfo/Immunology/Autoimmunedisease.aspx

DepartmentClinical Immunology
SampleBlood
TubeSerum Gel
Tube Picture
Collection ConditionsNo restrictions on collection conditions HOWEVER please note that this test is not appropriate for pleural fluid samples.
Min. Vol2 mL
Freq.Weekdays

Ref. Range (Male)See notes
Ref. Range (Female)
Ref. Range (Paed)
Ref. Range NotesANA screen: Positive or negative. The ANA screen will be reported as positive if the patient is positive for one or more of the 12 specificic autoantigens. Positive anti-DNA Ab = 10IU/mL or above. Positive anti-ENA Ab - 1AI or above, execpt anti-La and -Scl-70 = 2.5AI or above. ANA by IIF is reported as POS/WEAK/NEG
Units- See comments
IP Acute TAT- Contact Laboratory
IP Routine TAT7 days
GP Acute TAT- Contact Laboratory
GP Routine TAT7 days
Turnround CommentMinimum re-test interval = 20 days

Originally edited by : BD. Review due on 15/02/2018 15:05:46. Published By AM on 15/02/2017 15:05:46.