|Description||The PFA 100 is an instrument and test cartridge system in which the process of platelet adhesion and aggregation following vascular injury is simulated in vitro. Platelet dysfunction detected by the PFA-100 system may be acquired, inherited, or induced by platelet inhibiting agents. The most common causes of platelet dysfunction are related to uraemia, von Willebrand disease (VWD), and exposure to agents, such as acetyl salicylic acid (ASA, for example Aspirin).|
|Indication||Bleeding diatheses where the indication may be due to irregular platelet function.|
|Additional Info||Usually requested as part of a full platelet function study. See also Platelet Function Tests.|
|Concurrent Tests||Platelet Aggregation Studies, Platelet Glycoproteins, Platelet Nucleotides, Full Blood Count, Blood film.|
|Interpretation||Increased closure times to both Coll/Epi and Coll/ADP cartridge would indicate a platelet function defect (results affected by low haematocrit and platelet count). Increased closure time to Coll/Epi cartridge only would indicate a drug induced platelet function defect.|
|Collection Conditions||NO antibiotics, aspirin or aspirin related products for 2 weeks prior to testing.
State if patient is taking anti-platelet therapy.
3.5 ml citrated whole blood (2 blue Greiner tube or 4 paediatric dark blue Sarstedt 1.3ml tubes) is required for the analysis.
0.8 ml of blood is required for each test cartridge performed in duplicate.
Venepuncture should be performed using a 21G or larger needle (20g or 19G).
Samples must be stored at room temperature, undisturbed and are stable for up to 4 hours.
N.B. The vacuum tube system must NOT be used to transport samples.|
|Min. Vol||4mL citrated whole blood|
|Ref. Range (Male)|
|Ref. Range (Female)|| |
|Ref. Range (Paed)|| |
|Ref. Range Notes||Male - Col/EPI = 85 – 165 secs
Col/ADP = 60 – 120 secs. Female - Col/EPI = 85 – 165 secs
Col/ADP = 60 – 120 secs|
|IP Acute TAT||4 weeks|
|IP Routine TAT||4 weeks|
|GP Acute TAT||4 weeks|
|GP Routine TAT||4 weeks|
Originally edited by : na. Review due on 10/01/2020 10:13:49. Published By on 10/01/2019 10:13:49.