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ID:1050 PFA-100
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Description**PLEASE NOTE - FROM JUNE 2019 THIS ASSAY WILL NO LONGER BE AVAILABLE** The PFA 100 is an instrument and test cartridge system in which the process of platelet adhesion and aggregation following vascular injury is simulated in vitro. Platelet dysfunction detected by the PFA-100 system may be acquired, inherited, or induced by platelet inhibiting agents. The most common causes of platelet dysfunction are related to uraemia, von Willebrand disease (VWD), and exposure to agents, such as acetyl salicylic acid (ASA, for example Aspirin).
IndicationBleeding diatheses where the indication may be due to irregular platelet function.
Additional InfoUsually requested as part of a full platelet function study. See also Platelet Function Tests.
Concurrent TestsPlatelet Aggregation Studies, Platelet Glycoproteins, Platelet Nucleotides, Full Blood Count, Blood film.
Dietary Requirementsna
InterpretationIncreased closure times to both Coll/Epi and Coll/ADP cartridge would indicate a platelet function defect (results affected by low haematocrit and platelet count). Increased closure time to Coll/Epi cartridge only would indicate a drug induced platelet function defect.

DepartmentSpecialist Coagulation
Tube Picture
Collection ConditionsNO antibiotics, aspirin or aspirin related products for 2 weeks prior to testing. State if patient is taking anti-platelet therapy. 3.5 ml citrated whole blood (2 blue Greiner tube or 4 paediatric dark blue Sarstedt 1.3ml tubes) is required for the analysis. 0.8 ml of blood is required for each test cartridge performed in duplicate. Venepuncture should be performed using a 21G or larger needle (20g or 19G). Samples must be stored at room temperature, undisturbed and are stable for up to 4 hours. N.B. The vacuum tube system must NOT be used to transport samples.
Min. Vol4mL citrated whole blood

Ref. Range (Male)
Ref. Range (Female)
Ref. Range (Paed)
Ref. Range NotesMale - Col/EPI = 85 165 secs Col/ADP = 60 120 secs. Female - Col/EPI = 85 165 secs Col/ADP = 60 120 secs
IP Acute TAT4 weeks
IP Routine TAT4 weeks
GP Acute TAT4 weeks
GP Routine TAT4 weeks
Turnround CommentNA

Originally edited by : na. Review due on 13/06/2020 14:53:30. Published By on 13/06/2019 14:53:30.