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ID:10 Alkaline Phosphatase Isoenzymes
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DescriptionIn cases where ALP activity is elevated and the source is not clear, ALP isoenzymes can be separated by gel electrophoresis to help determine the source.
IndicationRequested in order to determine the source of elevated ALP if not clear. May be of use for possible cases of benign transient hyperphosphatasia of infancy and childhood.
Additional InfoThe sample will only be referred for analysis if ALP > 150 iU/L. Requests for isoenzyme analysis with borderline high ALP values may be cancelled at the discretion of the Duty Biochemist. If GGT is raised, this strongly suggests a liver source for ALP and the sample may not be referred for isoenzyme analysis.
Concurrent Testsna
Dietary RequirementsFasting sample preferred
InterpretationWhen ALP is within the reference range, liver and bone fractions are present in approximately equal proportions. Elevated bone ALP may be due to vitamin D deficiency, hyperparathyroidism, Paget's disease, healing fractures or malignancy. Elevated liver ALP indicates cholestasis (various causes). Increased intestinal ALP can occur in non-fasting healthy individuals with blood group O or B, in diabetes mellitus and a variety of other conditions. Increased placental ALP is normal in pregnancy.

TubeSerum Gel
Tube Picture
Collection ConditionsFasting sample preferred
Min. Vol0.5mL

Ref. Range (Male)
Ref. Range (Female)
Ref. Range (Paed)
Ref. Range Notes
IP Acute TAT2 weeks
IP Routine TAT2 weeks
GP Acute TAT2 weeks
GP Routine TAT2 weeks
Turnround CommentSample sent to York for analysis

Originally edited by : EM. Review due on 13/08/2019 15:01:33. Published By Eleanor McLaughlan on 13/08/2018 15:01:33.