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ID:914 Eosin 5-Maleimide (EMA) binding test
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DescriptionEosin 5-Maleimide is a fluorescent dye that reacts to bind covalently to band 3 protein in the red cell membrane. Analysis by flow cytometry measures the fluorescent intensity of labelled intact red cells. This method is now accepted as the gold standard for the diagnosis of Hereditary Spherocytosis, especially in the neonatal period where equivocal results can be achieved using the current range of tests.
IndicationInvestigation of a suspected case of Hereditary Spherocytosis where the FBC result and morphology are indicative and other causes of haemolysis have been ruled out.
Additional InfoExamination of a blood film for the presence of spherocytes along with a clinical history is important in the evaluation of a reduced EMA binding test result. The presence of spherocytes and a reduced Eosin 5-Maleimide binding test result is suggestive of a diagnosis of Hereditary Spherocytosis. In some patients the test may not prove to be diagnostic and a diagnosis can only be made using other methods.
Concurrent TestsFBC, Film
Dietary Requirements

InterpretationProvided by laboratory

Ref. Range (Male)
Ref. Range (Female)
Ref. Range (Paed)
Ref. Range NotesThe laboratory MUST be contacted to arrange the test ( 0113 206 7034 ). Samples sent without prior arrangement may not be tested.
Units- Descriptive Report

Other Help:National Laboratory Medicine Handbook

IP Acute TAT- Not Available -
IP Routine TAT1 day
GP Acute TAT- Contact Laboratory
GP Routine TAT- Contact Laboratory
Turnround CommentThe laboratory MUST be contacted to arrange the test ( 0113 206 7034 ). Samples sent without prior arrangement may not be tested.
Originally edited by : Stephen Leather. Last edited on 30/07/2019 09:16:14. Published By Matthew Clifford on 30/07/2018 09:16:14.