Immunology News

Change to the Reference range and interpretation of Voltage-gated Potassium Channel (VGKC) Antibodies (28.10.16)

 

With effect from Friday 28/10/16, the reference range and interpretation of Voltage-gated Potassium Channel (VGKC) Antibodies will change to

  <69pmol/L Negative

  70-130pmol/L Equivocal

  >130pmol/L Positive

  Previously, results <100pm were interpreted as negative

  If you have any queries please contact Dr Sinisa Savic (Consultant Clinical Immunologist) x65567 or Anna McHugh (Clinical Scientist) x25550

 

Change to Reporting of anti-Tissue Transglutaminase antibody (Coeliac Screen) Results (19.5.14)

The Joint BSPGHAN and Coeliac UK guidelines for the diagnosis and management of coeliac disease in children were published in 2013.  To facilitate the use of these guidelines anti-tTG Ab results will be reported as a numerical value rather than as an interpretive result.  This change affects results for anti-tTG Ab for both paediatric and adult requests.  This change will be introduced from Monday 2nd June 2014.

Reference: Arch Dis Child 2013;98:806-811 Murch S, Jenkins H, Auth M, et al. 

The policy for testing for coeliac disease remains unchanged for both paediatric and adult patients: 

v Serum from patients with suspected coeliac disease will be assayed for IgA anti-tissue transglutaminase (tTg).  Anti-tTG Ab are detected on the Bioplex 2200 multiplex platform.  An IgA verification bead is included in this assay and is therefore able to detect patients who are IgA deficient which makes the requesting of IgA unnecessary.

 §  For all individuals over 6 years:

¨     Where only immunoglobulins are requested in addition to coeliac serology, IgA tTG ONLY will be performed as the first line test

¨     If this result raises the possibility of IgA deficiency, measurement of IgA will be undertaken, in addition to measurement of IgG endomysial antibodies.

§  For all individuals under 6 years:

¨     In addition to coeliac serology, immunoglobulin measurement will be performed.

¨     If the result identifies IgA deficiency measurement of IgG endomysial antibodies will be performed. 

v Samples positive for anti-tTg will be further tested for IgA anti-endomysial antibodies.

v Samples flagged on the Bioplex analyser to be potentially IgA deficient (fail the IgA verification bead test) will have immunoglobulin levels measured by nephelometry in order to detect IgA deficiency.

v Samples shown to be IgA deficient will be assayed for IgG anti-endomysial antibodies.  

v Requests for anti-gliadin antibodies will receive IgA anti-tTg.

 


Page updated: 19/04/18 | Updated by: Anna McHugh

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