Hormone Tests Requirements
Test Method Preferred sample type Alternative sample type Vol (mL) Min vol (mL)
17-OH Progesterone LC-MS/MS since June 2015 Serum Plasma 1 0.2
17-OH Progesterone in saliva in house RIA Saliva none 2 1
25-OH vitamin D2 D3 (1) Siemens Advia Centaur & (2) LC-MS/MS since Sept 2005 Serum Plasma (Li Hep or EDTA) 1 0.2
ACTH Immulite 2000 EDTA none 2 0.5
Aldosterone in house RIA Serum Plasma (Li Hep or EDTA) 1 0.3
Androstenedione LC-MS/MS since Jan 2007 Serum Plasma 1 0.2
NT pro BNP Immulite 2000 Heparin (preferably without gel) None 1 0.3
Cortisol Siemens Advia Centaur Serum Plasma 1 0.25
DHEA-sulphate in house RIA Serum Plasma 1 0.3
Dihydrotestosterone (DHT) in house RIA Serum Plasma 2.5

2.5

1.5
1.5

1.5

0.75
Gonadotrophins Siemens Advia Centaur Serum Plasma 1 0.5
Growth Hormone (GH 22kDa)
Immulite 2000 Serum Plasma 1 0.5
Insulin-like Growth Factor-1 (IGF-1) Immulite 2000 Serum none 2 1
Oestradiol (1) Siemens Advia Centaur & (2) in house RIA Serum Plasma 1 0.5
Plasma renin activity in house RIA Li Hep Plasma EDTA 1 0.3
Parathyroid hormone (PTH) Siemens Advia Centaur EDTA none 1 0.3
Sex hormone binding globulin (SHBG) Immulite 2000 Serum Plasma 1 0.3
Testosterone (1) Siemens Advia Centaur & (2) LC-MS/MS since May 2004 Serum Plasma 1 0.2
Thyroglobulin/ Thyroglobulin Antibodies Immulite 2000 Serum None 1 0.75
Thyroid hormones (free T4, total T3, TSH) Siemens Advia Centaur Serum Plasma  1
Urine free cortisol LC-MS/MS since Jan 2007 Plain urine 24 hour   10 0.5

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Page updated: 29/01/2016, | Updated by: Dr. Julian Barth