Hormone Tests Interpretation
TEST REFERENCE RANGES UNITS
17-OH Progesterone (in-house RIA) Adult males: 2 – 9; Adult females:          < 12
Neonates (>48 h after birth), unstressed: < 13
                                          stressed:    < 40
nmol/L
 
17-OH Progesterone in saliva (in-house RIA)
 
In 21-hydroxylase deficient patients showing adequate control:
08.00 h   650 – 2800
12.00 h   350 – 1700
16.00 h   220 – 1500
22.00 h   170 – 1100
pmol/L
25-OH vitamin D2 D3 (LC-MS/MS)
Total 25OH vitamin D2 D3
< 30 nmol/L suggests Vitamin D deficiency
30 – 75 nmol/L suggests depletion & may be insufficient to maintain skeletal health
> 75 nmol/L suggests Vitamin D sufficiency
> 500 nmol/L suggests toxicity
nmol/L
ACTH (Immulite 2000) 09.00 h: < 47
ng/L
Aldosterone (in-house RIA)
Na intake 100-150, K intake 50-100 mmol/day,
Adults aged 20-40 yrs:
08.00h after overnight recumbency; 100-450.
Random samples taken during normal activities through the day; 100-850.
Values decrease significantly in the elderly (> 60 yrs).
Values can be very high (>5000) in the neonate, but reduce rapidly.  Adult ref. ranges are reached by age 10 yrs
pmol/L
Androstenedione (LC-MS/MS)
Pre-adrenarche, male & female: < 1.4
Adult male: 1.3 – 5.8
Adult female: 1.1 – 5.7
nmol/L
NT proBNP
LOW result <400 ng/L. BNP indicates low probability of Cardiac Failure. Consider alternative diagnoses.
>INTERMEDIATE result 401-1999 ng/L. BNP is associated with heart failure in fewer than 25% of patients. Please review the patient and if heart failure is still suspected consider a referral for BNP-guided echocardiography
HIGH result >2000 ng/L. BNP is associated with a 50% chance of heart failure, and patients with this level should be referred to the heart failure clinic.
ng/L
Cortisol (Bayer Advia Centaur)
09.00 h: 150 – 600
24.00 h: < 50
nmol/L
DHEA-sulphate (in-house RIA)
 
Pre adrenarche: < 1
Age Range                    Females                Males
Post puberty – 24 y        2.7 – 11                3.6 – 13
                25 – 34 y       2.1 – 10                2.9 – 12
                35 – 49 y       1.3 – 8.5               1.7 – 10
                50 – 59 y       1.0 – 7.0               1.0 – 8.0
                60 – 69 y         < 6.0                      < 7.0
                    > 70 y         < 5.0                      < 6.0
µmol/L
Dihydrotestosterone (DHT) (in-house RIA)
Paediatric samples
Adult females:  0.2 - 1.0
Adult males:     0.9 - 2.9
nmol/L
Growth Hormone (GH) – 22kDa (Immulite 2000)
Dynamic stimulatory or inhibitory tests are used for the diagnosis of GH deficiency or excess.
Random measurements of GH are difficult to interpret.
µg/L
Gonadotrophins (Bayer Advia Centaur) Children: LH < FSH and both < 2
Males: LH and FSH 1 - 9
Females: follicular & luteal phases: LH 1 - 10, FSH 1 - 8
Females: post menopausal: LH and FSH > 30
iu/L
Insulin-like Growth Factor-1 (IGF-1) (Immulite 2000)
 
Age related reference ranges:
Age           Females & males      Age          Females & males       
1-7y           6.7-39                      18 y           21-76
  8 y           8.3-45                      19 y           18-63
  9 y           9.6-50                      20 y           17-55
10 y           11-59                       21-30 y      15-45
11 y           14-72                       31-40 y      15-40
12 y           19-90                       41-45 y      13-35
13 y           24-111                     46-50 y      12-33
14 y           29-126                     51-55 y      11-31
15 y           31-129                     56-65 y      10-28
16 y           29-117                     66-75 y        9-25
17 y           25-95                       76-85 y        7-22
Interference from haemolysis
nmol/L
Oestradiol (in-house RIA)
Prepubertal children:     < 30
Males:                         < 150
Females:
Pre menopausal           200 – 2000
Post menopausal         < 150
pmol/L
Oestrone (Note: this assay not currently available)
 
Provisional  ref. ranges:
Adult females: pre menopausal     100 – 600
                      post menopausal         < 240
Adult males:                                      < 330
pmol/L
Parathyroid hormone (PTH) (Bayer Advia Centaur)  1.5 - 7.6 pmol/L 
Plasma Renin Activity  (in-house RIA of angiotensin generates)
Affected by age, posture, state of hydration & electrolyte status.
Na intake 100 – 150, K intake 50 – 100 mmol/day
Adults aged 20 – 40 y:
08.00 h after overnight recumbency                1.1 – 2.7
08.30 h after 30 min ambulant                       2.8 – 4.5
Samples taken at random during the
day & normal activities                                  0.5 – 3.5
Values can be very high (>50) in the neonate & decrease in the elderly.
nmol/L/h
SHBG  (Immulite 2000)
Adult female:  18 -114
Adult male:     13 - 71
nmol/L
Testosterone (LC-MS/MS)
Age & sex-related ranges:
Female:               Birth – 8 y              < 0.9
                           Adult                      < 1.8
Male:                   6 month – 8 y         < 0.9
                           Adult                      8 – 30
nmol/L
Thyroglobulin/Thyroglobulin Antibodies (Immulite 2000)
Thyroglobulin should be undetectable in patients with thyroid cancer treated with total thyroidectomy and radio-iodine therapy.
 
The presence of thyroglobulin autoantibodies (TgAb) can interfere with the thyroglobulin assay, hence the TgAb status of the patient is measured on all samples. In addition, TgAb may act as a surrogate tumour markers after total thyroidectomy.
µg/L
iu/mL
Thyroid hormones  (Bayer Advia Centaur) free T4    10-20
total T3    0.9-2.5
TSH        0.2-4.0
pmol/L
nmol/L
miu/L
Urine Free Cortisol (LC-MS/MS)
Age related ranges:
Male & female,       5 – 10 y      contact lab
                            10 – 15 y     contact lab
                            15 – 70 y     < 147
nmol/24 h

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Page updated: 21/02/2017 | Updated by: Dr. Julian Barth