July 27, 2017
Lipoprotein lipase deficiency Minimize

Protocol for heparin-released plasma lipase activity

Indication

Chylomicronaemia or primary hypertriglyceridaemia not due to type III hyperlipidaemia (usually due to Apo E 2:2).
 

Contraindication

This test should not be performed in individuals on aspirin, with a history of bleeding diathesis or sensitivity to heparin, nor with a history of CVA, rheumatic fever or if there is a peptic ulcer.
 

Principle

Heparin released lipoprotein lipase from fat and muscle.
 

Preparation

  • Patient should have fasted overnight
  • Patient should have refrained from alcohol for 24 hours
  • Patient should have refrained from heavy exercise for 48 hours

Requirements

  1. iv cannula and saline flush
  2. heparin 60 IU/kg body weight
  3. EDTA blood tubes for 20 mL
  4. 2 Li Heparin tubes for 10 mL
  5. ice to transport samples to laboratory

Procedure

  1. insert iv cannula
  2. collect 20 mL blood into EDTA tubes
  3. time -5 min: collect 10 mL blood into Li Heparin tubes
  4. time 0 min: inject heparin 60 IU/kg body weight as iv bolus and flush line with 10 mL saline
  5. time 10 min: collect 10 mL blood into Li Heparin tubes

Laboratory Sample Handling

Samples should be transported to the laboratory on ice and separated. All plasma samples should be split into 4 aliquots and frozen at -20C. All the samples should be identified by time and matrix ie Li Hep or EDTA, and should be transported to laboratory on dry ice for measurement of lipoprotein lipase and apolipoprotein CII.

Interpretation

Heterozygotes exhibit a 50% decrease of LPL enzyme activity in plasma following intravenous administration of heparin

Reference

Brunzell JD. Familial lipoprotein lipase deficiency. GeneReviews.

 
JHB  13 September 2009
  

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