Protocol for heparin-released plasma lipase activity
Chylomicronaemia or primary hypertriglyceridaemia not due to type III hyperlipidaemia (usually due to Apo E 2:2).
This test should not be performed in individuals on aspirin, with a history of bleeding diathesis or sensitivity to heparin, nor with a history of CVA, rheumatic fever or if there is a peptic ulcer.
Heparin released lipoprotein lipase from fat and muscle.
Patient should have fasted overnight
Patient should have refrained from alcohol for 24 hours
Patient should have refrained from heavy exercise for 48 hours
iv cannula and saline flush
heparin 60 IU/kg body weight
EDTA blood tubes for 20 mL
2 Li Heparin tubes for 10 mL
ice to transport samples to laboratory
insert iv cannula
collect 20 mL blood into EDTA tubes
time -5 min: collect 10 mL blood into Li Heparin tubes
time 0 min: inject heparin 60 IU/kg body weight as iv bolus and flush line with 10 mL saline
time 10 min: collect 10 mL blood into Li Heparin tubes
Laboratory Sample Handling
Samples should be transported to the laboratory on ice and separated. All plasma samples should be split into 4 aliquots and frozen at -20C. All the samples should be identified by time and matrix ie Li Hep or EDTA, and should be transported to laboratory on dry ice for measurement of lipoprotein lipase and apolipoprotein CII.
Heterozygotes exhibit a 50% decrease of LPL enzyme activity in plasma following intravenous administration of heparin
Brunzell JD. Familial lipoprotein lipase deficiency. GeneReviews.
JHB 13 September 2009